R\u00f6vid le\u00edr\u00e1s<\/strong><\/span>
A tal\u00e1lm\u00e1ny szerinti \u00faj molekul\u00e1ris kl\u00f3noz\u00e1si elj\u00e1r\u00e1s, melynek sor\u00e1n egy v\u00e1lasztott DNS fragmentumot (kl\u00f3nozand\u00f3 DNS szakasz vagy m\u00e1sk\u00e9ppen \u201einszert\u201d) hozz\u00e1adott restrikci\u00f3s endonukle\u00e1z, lig\u00e1z, DNS polimer\u00e1z, 5′ exonukle\u00e1z vagy egy\u00e9b enzim haszn\u00e1lata n\u00e9lk\u00fcl juttatnak be egy adott DNS vektorba, k\u00fcl\u00f6n\u00f6sen alkalmas \u00faj DNS konstrukci\u00f3k gyors \u00e9s olcs\u00f3 el\u0151\u00e1ll\u00edt\u00e1s\u00e1ra, mivel enzimek haszn\u00e1lata n\u00e9lk\u00fcl lehets\u00e9ges \u00e1ltala DNS kl\u00f3noz\u00e1st megval\u00f3s\u00edtani. Emiatt a tal\u00e1lm\u00e1ny szerinti megold\u00e1s kifejezetten el\u0151ny\u00f6sen alkalmazhat\u00f3 korl\u00e1tozott er\u0151forr\u00e1sokkal rendelkez\u0151 laborat\u00f3riumok sz\u00e1m\u00e1ra. A jelen megold\u00e1s tov\u00e1bbi el\u0151nye, hogy megk\u00f6nny\u00edti a hossz\u00fa DNS fragmentumok kl\u00f3noz\u00e1s\u00e1t, l\u00e9v\u00e9n nem ig\u00e9nyel specifikus restrikci\u00f3s endonukle\u00e1z has\u00edt\u00f3helyeket a vektor \u00e9s inszert DNS szakaszokon, amelyek k\u00fcl\u00f6n\u00f6sen a hossz\u00fa DNS fragmentumok kl\u00f3noz\u00e1sa eset\u00e9n problematikus.<\/p>\n[\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=”4.18.1″ custom_margin=”||0px||false|false” custom_padding=”||0px||false|false” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][et_pb_column_inner saved_specialty_column_type=”3_4″ _builder_version=”4.16″ global_colors_info=”{}”][et_pb_button button_url=”mailto:info@danubia.hu” button_text=”Kapcsolat” button_alignment=”left” module_class=”gq_r_fixed_buttons” _builder_version=”4.18.1″ custom_button=”on” button_text_size=”16″ button_text_color=”#ffffff” button_bg_color=”#a6192e” button_border_color=”#a6192e” button_font=”Raleway|600||on|||||” button_icon=”9||divi||400″ animation_style=”fade” hover_enabled=”0″ z_index_tablet=”500″ global_colors_info=”{}” sticky_enabled=”0″][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=”4.16″ custom_margin=”0px||||false|false” global_colors_info=”{}”][et_pb_column_inner saved_specialty_column_type=”3_4″ _builder_version=”4.16″ global_colors_info=”{}”][et_pb_button button_url=”https:\/\/www.danubia.com\/hu\/danubia-ipmall\/#piacter” button_text=”Vissza a szellemitulajdon-piact\u00e9rre” button_alignment=”left” module_class=”gq_r_fixed_buttons” _builder_version=”4.16″ custom_button=”on” button_text_size=”16″ button_text_color=”#a6192e” button_bg_color=”#ffffff” button_border_color=”#a6192e” button_font=”Raleway|600||on|||||” button_icon=”9||divi||400″ button_icon_color=”#a6192e” animation_style=”fade” z_index_tablet=”500″ global_colors_info=”{}” button_bg_color__hover_enabled=”on|desktop” button_bg_color__hover=”#ffffff”][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"
A tal\u00e1lm\u00e1ny szerinti \u00faj molekul\u00e1ris kl\u00f3noz\u00e1si elj\u00e1r\u00e1s, melynek sor\u00e1n egy v\u00e1lasztott DNS fragmentumot (kl\u00f3nozand\u00f3 DNS szakasz vagy m\u00e1sk\u00e9ppen \u201einszert\u201d) hozz\u00e1adott restrikci\u00f3s endonukle\u00e1z, lig\u00e1z, DNS polimer\u00e1z, 5′ exonukle\u00e1z vagy egy\u00e9b enzim haszn\u00e1lata n\u00e9lk\u00fcl juttatnak be egy adott DNS vektorba, k\u00fcl\u00f6n\u00f6sen alkalmas \u00faj DNS konstrukci\u00f3k gyors \u00e9s olcs\u00f3 el\u0151\u00e1ll\u00edt\u00e1s\u00e1ra, mivel enzimek haszn\u00e1lata n\u00e9lk\u00fcl lehets\u00e9ges \u00e1ltala DNS kl\u00f3noz\u00e1st megval\u00f3s\u00edtani.<\/p>\n","protected":false},"author":1,"featured_media":9556,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"
[et_pb_section fb_built=\"1\" specialty=\"on\" _builder_version=\"3.22\" inner_max_width=\"none\"][et_pb_column type=\"1_4\" _builder_version=\"3.25\" custom_padding=\"|||\" custom_padding__hover=\"|||\"][et_pb_image src=\"https:\/\/www.danubia.com\/wp-content\/uploads\/2021\/08\/Valos-ideju-fluoreszcencia-alapu-vizsgalat-az-OATP-transzporterek-funkcionalis-tesztelesehez-es-gyogyszer-kolcsonhatasainak-vizsgalatahoz.jpg\" title_text=\"Valos ideju fluoreszcencia alapu vizsgalat az OATP-transzporterek funkcionalis tesztelesehez es gyogyszer-kolcsonhatasainak vizsgalatahoz\" align_tablet=\"center\" align_phone=\"\" align_last_edited=\"on|desktop\" _builder_version=\"4.9.4\" custom_margin=\"||0px|\" custom_padding=\"||15px|\" animation_style=\"fade\" hover_enabled=\"0\" z_index_tablet=\"500\" sticky_enabled=\"0\"][\/et_pb_image][\/et_pb_column][et_pb_column type=\"3_4\" specialty_columns=\"3\" _builder_version=\"3.25\" custom_padding=\"|||\" custom_padding__hover=\"|||\"][et_pb_row_inner admin_label=\"Sor\" _builder_version=\"3.25\"][et_pb_column_inner saved_specialty_column_type=\"3_4\" _builder_version=\"3.25\" custom_padding=\"|||\" custom_padding__hover=\"|||\"][et_pb_text admin_label=\"N\u00e9v\" _builder_version=\"4.9.4\" text_font=\"Raleway|700||on|||||\" text_text_color=\"#a6192e\" text_font_size=\"26px\" text_line_height=\"1.3em\" header_font=\"Raleway|700||on|||||\" header_text_align=\"center\" header_text_color=\"#ffffff\" header_font_size=\"50px\" header_line_height=\"1.4em\" custom_margin=\"||0px|\" custom_padding=\"||15px|\" hover_enabled=\"0\" text_font_size_tablet=\"22px\" text_font_size_phone=\"20px\" text_font_size_last_edited=\"on|phone\" header_font_size_tablet=\"30px\" header_font_size_phone=\"22px\" header_font_size_last_edited=\"on|desktop\" z_index_tablet=\"500\" sticky_enabled=\"0\"]Val\u00f3s idej\u0171 fluoreszcencia alap\u00fa vizsg\u00e1lat az OATP-transzporterek funkcion\u00e1lis tesztel\u00e9s\u00e9hez \u00e9s gy\u00f3gyszer-k\u00f6lcs\u00f6nhat\u00e1sainak vizsg\u00e1lat\u00e1hoz[\/et_pb_text][et_pb_text admin_label=\"Bejelent\u00e9s \u00fcgysz\u00e1ma, Lajstromsz\u00e1m\" _builder_version=\"4.9.4\" text_font=\"Raleway|700|||||||\" text_text_color=\"#63666a\" text_font_size=\"18px\" custom_margin=\"||0px|\" custom_padding=\"||10px|\" hover_enabled=\"0\" text_font_size_tablet=\"20px\" text_font_size_phone=\"\" text_font_size_last_edited=\"on|desktop\" z_index_tablet=\"500\" sticky_enabled=\"0\"]<\/p>
Reprezentat\u00edv \u00fcgysz\u00e1m: WO\/2021\/123851
Oltalom: Szabadalom
Ipar\u00e1g: gy\u00f3gyszer \u00e9s kozmetikai ipar<\/p>
[\/et_pb_text][et_pb_divider divider_position=\"center\" divider_weight=\"3\" _builder_version=\"4.4.8\" max_width=\"50px\" module_alignment=\"left\" height=\"0px\" animation_style=\"zoom\" z_index_tablet=\"500\"][\/et_pb_divider][et_pb_text admin_label=\"Le\u00edr\u00e1s\" _builder_version=\"4.9.4\" text_orientation=\"justified\" hover_enabled=\"0\" z_index_tablet=\"500\" sticky_enabled=\"0\"]<\/p>
R\u00f6vid le\u00edr\u00e1s<\/strong><\/span>
A tal\u00e1lm\u00e1ny az OATP (szerves aniont sz\u00e1ll\u00edt\u00f3 polipeptid) transzportfeh\u00e9rj\u00e9k transzportaktivit\u00e1s\u00e1nak \u00e9rt\u00e9kel\u00e9s\u00e9re, k\u00fcl\u00f6n\u00f6sen egy val\u00f3s idej\u0171, add-and-read fluoreszcencia-alap\u00fa elj\u00e1r\u00e1sra vonatkozik, mely annak meghat\u00e1roz\u00e1s\u00e1ra szolg\u00e1l, miszerint a tesztvegy\u00fclet befoly\u00e1solja-e az OATP szubsztr\u00e1ttranszport-aktivit\u00e1s\u00e1t. Az elj\u00e1r\u00e1s egy nem fluoreszcens kontroll szubsztr\u00e1t alkalmaz\u00e1s\u00e1val val\u00f3sul meg, amely a sejtben fluoreszcens tulajdons\u00e1got mutat fel. A m\u00f3dszer alkalmazhat\u00f3 az OATP expresszi\u00f3j\u00e1nak m\u00e9r\u00e9s\u00e9re \u00e9s a sz\u00e1ll\u00edtott szubsztr\u00e1t valamint az OATP inhibitor k\u00f6z\u00f6tti k\u00fcl\u00f6nbs\u00e9gt\u00e9telre is.<\/p>[\/et_pb_text][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner column_structure=\"1_2,1_2\" _builder_version=\"4.4.8\" custom_margin=\"||0px||false|false\" custom_padding=\"||0px||false|false\"][et_pb_column_inner type=\"1_2\" saved_specialty_column_type=\"3_4\" _builder_version=\"4.4.8\"][et_pb_button button_url=\"https:\/\/patentscope.wipo.int\/search\/en\/detail.jsf?docId=WO2021123851\" url_new_window=\"on\" button_text=\"Tov\u00e1bbi inform\u00e1ci\u00f3k\" button_alignment=\"left\" module_class=\"gq_r_fixed_buttons\" _builder_version=\"4.9.4\" custom_button=\"on\" button_text_size=\"16\" button_text_color=\"#ffffff\" button_bg_color=\"#a6192e\" button_border_color=\"#a6192e\" button_font=\"Raleway|600||on|||||\" button_icon=\"%%24%%\" animation_style=\"fade\" hover_enabled=\"0\" z_index_tablet=\"500\" sticky_enabled=\"0\"][\/et_pb_button][\/et_pb_column_inner][et_pb_column_inner type=\"1_2\" saved_specialty_column_type=\"3_4\" _builder_version=\"4.4.8\"][et_pb_button button_url=\"mailto:info@danubia.hu\" button_text=\"Kapcsolatfelv\u00e9tel\" button_alignment=\"right\" module_class=\"gq_r_fixed_buttons\" _builder_version=\"4.9.4\" custom_button=\"on\" button_text_size=\"16\" button_text_color=\"#ffffff\" button_bg_color=\"#a6192e\" button_border_color=\"#a6192e\" button_font=\"Raleway|600||on|||||\" button_icon=\"%%24%%\" animation_style=\"fade\" z_index_tablet=\"500\"][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=\"4.4.8\" custom_margin=\"0px||||false|false\"][et_pb_column_inner saved_specialty_column_type=\"3_4\" _builder_version=\"4.4.8\"][et_pb_button button_url=\"https:\/\/www.danubia.com\/hu\/danubia-ipmall\/#piacter\" button_text=\"Vissza a szellemitulajdon-piact\u00e9rre\" button_alignment=\"left\" module_class=\"gq_r_fixed_buttons\" _builder_version=\"4.4.8\" custom_button=\"on\" button_text_size=\"16\" button_text_color=\"#a6192e\" button_bg_color=\"#ffffff\" button_border_color=\"#a6192e\" button_font=\"Raleway|600||on|||||\" button_icon=\"%%24%%\" button_icon_color=\"#a6192e\" animation_style=\"fade\" z_index_tablet=\"500\" button_bg_color__hover_enabled=\"on|desktop\"][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section]<\/p>","_et_gb_content_width":"","footnotes":""},"project_category":[161,178,172,186],"project_tag":[],"class_list":["post-9554","project","type-project","status-publish","has-post-thumbnail","hentry","project_category-biotechnologia","project_category-biotechnology","project_category-gyogyszeripar-es-kozmetikai-ipar","project_category-pharmaceutical-and-cosmetics-industry"],"yoast_head":"\n
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