Method for DNA cloning without using in vitro enzymatic reaction, and the sequences of related DNA vectors

Method for DNA cloning without using in vitro enzymatic reaction, and the sequences of related DNA vectors

The current novel molecular DNA cloning procedure – during which a given DNA fragment (DNA stretch subjected to cloning, also called “insert”) aimed to be multiplied is introduced into a vector DNA (“vehicle”) without using in vitro added enzymes such as restriction endonucleases, ligases, DNA polymerases and 5′ exonucleases – is particularly suitable for generating novel DNA constructs in a relatively fast and cost-effective way, as it performs DNA cloning without using in vitro added enzymes.

Synthesis of novel imipridone derivatives and their evaluation for their anticancer activity

Synthesis of novel imipridone derivatives and their evaluation for their anticancer activity

Applying novel imipridone derivatives, containing 3,5-disubstituted benzyl group attached to position 7, being subject of the present invention, especially on patients suffering from prostate carcinoma, pancreatic cancer, lung carcinoma, breast cancer, glioma, cancers of head and neck, colon cancer, skin cancer proved to show enhanced anticancer activity.

A real-time, add-and-read fluorescence-based assay for testing function and drug interactions of OATP transporters

A real-time, add-and-read fluorescence-based assay for testing function and drug interactions of OATP transporters

The invention relates to the field of assessing transport activity of OATP (Organic anion-transporting polypeptide) transport proteins. In particular, the invention relates to a real-time, add-and-read fluorescence-based method for determining whether a test compound interferes with substrate transport activity of an OATP, by using a non-fluorescent control substrate which becomes fluorescent within a cell. The method is also applicable to measure the expression of an OATP and to discriminate between a transported substrate and an inhibitor of an OATP.

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DANUBIA
DANUBIA IP
DANUBIA LEGAL